Abstract

Plasmodium falciparum: a method for the amplification of S antigens and its application to laboratory and field samples

Kyes, S. Craig, A. G. Marsh, K. Newbold, C. I.
Exp Parasitol. 1993; 77473-83

Permanent descriptor

https://doi.org/10.1006/expr.1993.1108

Polymerase chain reaction amplification of several polymorphic genes has been used to study the population biology of Plasmodium falciparum. S antigen is particularly suitable for such studies, but difficulties in the amplification of this gene have precluded its use. Here we describe a simple method for the amplification of S antigen and show why previous attempts may have been unsuccessful. Data are presented from both laboratory and field isolates.