Proteomic analysis of extracellular vesicles from a Plasmodium falciparum Kenyan clinical isolate defines a core parasite secretome
Wellcome Open Res
BACKGROUND: Many pathogens secrete effector molecules to subvert host immune responses, to acquire nutrients, and/or to prepare host cells for invasion. One of the ways that effector molecules are secreted is through extracellular vesicles (EVs) such as exosomes. Recently, the malaria parasite P. falciparum has been shown to produce EVs that can mediate transfer of genetic material between parasites and induce sexual commitment. Characterizing the content of these vesicles may improve our understanding of P. falciparum pathogenesis and virulence. METHODS: Previous studies of P. falciparum EVs have been limited to long-term adapted laboratory isolates. In this study, we isolated EVs from a Kenyan P. falciparum clinical isolate adapted to in vitro culture for a short period and characterized their protein content by mass spectrometry (data are available via ProteomeXchange, with identifier PXD006925). RESULTS: We show that P. falciparum extracellular vesicles ( PfEVs) are enriched in proteins found within the exomembrane compartments of infected erythrocytes such as Maurer’s clefts (MCs), as well as the secretory endomembrane compartments in the apical end of the merozoites, suggesting that these proteins play a role in parasite-host interactions. Comparison of this novel clinically relevant dataset with previously published datasets helps to define a core secretome present in Plasmodium EVs. CONCLUSIONS: P. falciparum extracellular vesicles contain virulence-associated parasite proteins. Therefore, analysis of PfEVs contents from a range of clinical isolates, and their functional validation may improve our understanding of the virulence mechanisms of the parasite, and potentially identify targets for interventions or diagnostics.
Abdi, A., Yu, L., Goulding, D., Rono, M. K., Bejon, P., Choudhary, J., Rayner, J.
Pages:50, Volume:2, Edition:9/26/2017, Date,
Notes:Abdi, Abdirahman|Yu, Lu|Goulding, David|Rono, Martin K|Bejon, Philip|Choudhary, Jyoti|Rayner, Julian|eng|G1002624/Medical Research Council/United Kingdom|England|2017/09/26 06:00|Wellcome Open Res. 2017 Nov 22;2:50. doi: 10.12688/wellcomeopenres.11910.2. eCollection 2017.
ISBN: 2398-502X (Print)|2398-502X (Linking) Permanent ID: PMC5583745 Accession Number: 28944300
Author Address: Pwani University Bioscience Research Centre, Pwani University, Kilifi, Kenya.|KEMRI-Wellcome Trust Research Programme, Kilifi, Kenya.|Wellcome Trust Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, UK.