J Clin Virol
Many SARS-CoV-2 antibody detection assays have been developed but their differential performance is not well described. In this study we compared an in-house (KWTRP) ELISA which has been used extensively to estimate seroprevalence in the Kenyan population with WANTAI, an ELISA which has been approved for widespread use by the WHO. Using a wide variety of sample sets including pre-pandemic samples (negative gold standard), SARS-CoV-2 PCR positive samples (positive gold standard) and COVID-19 test samples from different periods (unknowns), we compared performance characteristics of the two assays. The overall concordance between WANTAI and KWTRP was 0.97 (95% CI, 0.95-0.98). For WANTAI and KWTRP, sensitivity was 0.95 (95% CI 0.90-0.98) and 0.93 (95% CI 0.87-0.96), respectively. Specificity for WANTAI was 0.98 (95% CI, 0.96-0.99) and 0.99 (95% CI 0.96-1.00) while KWTRP specificity was 0.99 (95% CI, 0.98-1.00) and 1.00 using pre-pandemic blood donors and pre-pandemic malaria cross-sectional survey samples respectively. Both assays show excellent characteristics to detect SARS-CoV-2 antibodies.
Nyagwange, J., Kutima, B., Mwai, K., Karanja, H. K., Gitonga, J. N., Mugo, D., Uyoga, S., Tuju, J., Ochola-Oyier, L. I., Ndungu, F., Bejon, P., Agweyu, A., Adetifa, I. M. O., Scott, J. A. G., Warimwe, G. M.
Pages:105061, Volume:146, Edition:1/2/2022, Date,Jan
Notes:Nyagwange, James|Kutima, Bernadette|Mwai, Kennedy|Karanja, Henry K|Gitonga, John N|Mugo, Daisy|Uyoga, Sophie|Tuju, James|Ochola-Oyier, Lynette I|Ndungu, Francis|Bejon, Philip|Agweyu, Ambrose|Adetifa, Ifedayo M O|Scott, J Anthony G|Warimwe, George M|eng|WT_/Wellcome Trust/United Kingdom|Research Support, Non-U.S. Gov’t|Netherlands|2022/01/02 06:00|J Clin Virol. 2022 Jan;146:105061. doi: 10.1016/j.jcv.2021.105061. Epub 2021 Dec 27.
ISBN: 1873-5967 (Electronic)|1386-6532 (Linking) Permanent ID: PMC8711170 Accession Number: 34973474
Author Address: KEMRI-Wellcome Trust Research Programme, PO Box 230, Kilifi, Kenya. Electronic address: email@example.com.|KEMRI-Wellcome Trust Research Programme, PO Box 230, Kilifi, Kenya.|KEMRI-Wellcome Trust Research Programme, PO Box 230, Kilifi, Kenya| School of Public Health, Faculty of Health Sciences, University of the Witwatersrand, 27 St Andrews Road, Parktown 2193, Johannesburg, South Africa.|KEMRI-Wellcome Trust Research Programme, PO Box 230, Kilifi, Kenya| Nuffield Department of Medicine, Oxford University, OX3 7BN, Oxford, United Kingdom.|KEMRI-Wellcome Trust Research Programme, PO Box 230, Kilifi, Kenya| Department of Infectious Diseases Epidemiology, London School of Hygiene and Tropical Medicine, WC1E 7HT, Keppel Street, London, United Kingdom.