Title :

Serological Profiling for Malaria Surveillance Using a Standard ELISA Protocol.

Abstract :

The enzyme-linked immunosorbent assay (ELISA) is a reliable and relatively low-cost method for measuring soluble ligands such as antibodies and proteins in biological samples. For analysis of specific antibodies in serum, a capture antigen is immobilized onto a solid polystyrene surface from which it can capture the antibodies. The captured antibodies are subsequently detected using a secondary antibody conjugated to an enzyme. Detection is accomplished by addition of a colorimetric substrate, and the readout is absorbance (optical density). Here, we provide a detailed standardized ELISA protocol for the quantification of antibodies against malaria antigens.

Authors :

Murungi, L.M., Kimathi, R.K., Tuju, J., Kamuyu, G., Osier, F.H.A.

PubMed link :

Journals :

Methods in Molecular Biology. 2019